glosensor-22f camp plasmid dna Search Results


glosensor camp reagent  (Promega)
90
Promega glosensor camp reagent
Glosensor Camp Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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glosensor 22f plasmid  (Promega)
90
Promega glosensor 22f plasmid
Glosensor 22f Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor-22f+camp+plasmid+dna/pglosensor+22f+camp+plasmid/pm40537032-161-16-19
Average 90 stars, based on 1 article reviews
glosensor 22f plasmid - by Bioz Stars, 2026-10
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profections mammalian transfection kit  (Promega)
90
Promega profections mammalian transfection kit
Profections Mammalian Transfection Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
profections mammalian transfection kit - by Bioz Stars, 2026-10
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camp biosensor glosensor-22f  (Promega)
90
Promega camp biosensor glosensor-22f
Camp Biosensor Glosensor 22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
camp biosensor glosensor-22f - by Bioz Stars, 2026-10
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glosensor luciferase-based assay  (Promega)
90
Promega glosensor luciferase-based assay
Nalfurafine is a potent G protein-biased KOR agonist. Comparison of G protein and β-arrestin signaling outcomes with a variety of KOR ligands (at indicated concentrations) compared with the reference standard U50,488. (A) G protein signaling was assessed with a <t>GloSensor</t> assay of cyclic AMP inhibition. (B) β-arrestin recruitment to the activated KOR was assessed via the Tango assay. (C and D) Results from GloSensor and Tango assays used to calculate bias factors for each compound from observed maximal efficacy (Emax or “Max”) and potency (EC50) values. ∆∆log(Max/EC50) values, with their 95% confidence intervals (CIs), are plotted in (D) for each compound to indicate relative (to U50,488) bias toward G protein signaling. All compounds were tested in triplicate alongside the reference standard U50,488. N ≥ 3 for all compounds.
Glosensor Luciferase Based Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor-22f+camp+plasmid+dna/glosensor+luminescence+assay/pmc06863463-60-16-47
Average 90 stars, based on 1 article reviews
glosensor luciferase-based assay - by Bioz Stars, 2026-10
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wildtype human gpr52 plasmid cdna  (Promega)
90
Promega wildtype human gpr52 plasmid cdna
Nalfurafine is a potent G protein-biased KOR agonist. Comparison of G protein and β-arrestin signaling outcomes with a variety of KOR ligands (at indicated concentrations) compared with the reference standard U50,488. (A) G protein signaling was assessed with a <t>GloSensor</t> assay of cyclic AMP inhibition. (B) β-arrestin recruitment to the activated KOR was assessed via the Tango assay. (C and D) Results from GloSensor and Tango assays used to calculate bias factors for each compound from observed maximal efficacy (Emax or “Max”) and potency (EC50) values. ∆∆log(Max/EC50) values, with their 95% confidence intervals (CIs), are plotted in (D) for each compound to indicate relative (to U50,488) bias toward G protein signaling. All compounds were tested in triplicate alongside the reference standard U50,488. N ≥ 3 for all compounds.
Wildtype Human Gpr52 Plasmid Cdna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor-22f+camp+plasmid+dna/wildtype+human+gpr52+plasmid+cdna/10__1021_slash_acs__jmedchem__0c01498-651-14-26
Average 90 stars, based on 1 article reviews
wildtype human gpr52 plasmid cdna - by Bioz Stars, 2026-10
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transit cho reagent  (Mirus Bio)
96
Mirus Bio transit cho reagent
Nalfurafine is a potent G protein-biased KOR agonist. Comparison of G protein and β-arrestin signaling outcomes with a variety of KOR ligands (at indicated concentrations) compared with the reference standard U50,488. (A) G protein signaling was assessed with a <t>GloSensor</t> assay of cyclic AMP inhibition. (B) β-arrestin recruitment to the activated KOR was assessed via the Tango assay. (C and D) Results from GloSensor and Tango assays used to calculate bias factors for each compound from observed maximal efficacy (Emax or “Max”) and potency (EC50) values. ∆∆log(Max/EC50) values, with their 95% confidence intervals (CIs), are plotted in (D) for each compound to indicate relative (to U50,488) bias toward G protein signaling. All compounds were tested in triplicate alongside the reference standard U50,488. N ≥ 3 for all compounds.
Transit Cho Reagent, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor-22f+camp+plasmid+dna/TransIT+-PRO/pm34467758-177-21-23
Average 96 stars, based on 1 article reviews
transit cho reagent - by Bioz Stars, 2026-10
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iowa 1 n a b arrestin 2 myfp robert lefkowitz addgene plasmid  (Addgene inc)
93
Addgene inc iowa 1 n a b arrestin 2 myfp robert lefkowitz addgene plasmid
Nalfurafine is a potent G protein-biased KOR agonist. Comparison of G protein and β-arrestin signaling outcomes with a variety of KOR ligands (at indicated concentrations) compared with the reference standard U50,488. (A) G protein signaling was assessed with a <t>GloSensor</t> assay of cyclic AMP inhibition. (B) β-arrestin recruitment to the activated KOR was assessed via the Tango assay. (C and D) Results from GloSensor and Tango assays used to calculate bias factors for each compound from observed maximal efficacy (Emax or “Max”) and potency (EC50) values. ∆∆log(Max/EC50) values, with their 95% confidence intervals (CIs), are plotted in (D) for each compound to indicate relative (to U50,488) bias toward G protein signaling. All compounds were tested in triplicate alongside the reference standard U50,488. N ≥ 3 for all compounds.
Iowa 1 N A B Arrestin 2 Myfp Robert Lefkowitz Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor-22f+camp+plasmid+dna/beta-arrestin2+mYFP+(Plasmid+%2336917)/pm41722048-322-217-223
Average 93 stars, based on 1 article reviews
iowa 1 n a b arrestin 2 myfp robert lefkowitz addgene plasmid - by Bioz Stars, 2026-10
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bz-h4m software  (KEYENCE)
90
KEYENCE bz-h4m software
Nalfurafine is a potent G protein-biased KOR agonist. Comparison of G protein and β-arrestin signaling outcomes with a variety of KOR ligands (at indicated concentrations) compared with the reference standard U50,488. (A) G protein signaling was assessed with a <t>GloSensor</t> assay of cyclic AMP inhibition. (B) β-arrestin recruitment to the activated KOR was assessed via the Tango assay. (C and D) Results from GloSensor and Tango assays used to calculate bias factors for each compound from observed maximal efficacy (Emax or “Max”) and potency (EC50) values. ∆∆log(Max/EC50) values, with their 95% confidence intervals (CIs), are plotted in (D) for each compound to indicate relative (to U50,488) bias toward G protein signaling. All compounds were tested in triplicate alongside the reference standard U50,488. N ≥ 3 for all compounds.
Bz H4m Software, supplied by KEYENCE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor-22f+camp+plasmid+dna/bz+h1c+software/10__1016_slash_j__isci__2025__112420-285-19-47
Average 90 stars, based on 1 article reviews
bz-h4m software - by Bioz Stars, 2026-10
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pglosensor-22f  (Promega)
90
Promega pglosensor-22f
Nalfurafine is a potent G protein-biased KOR agonist. Comparison of G protein and β-arrestin signaling outcomes with a variety of KOR ligands (at indicated concentrations) compared with the reference standard U50,488. (A) G protein signaling was assessed with a <t>GloSensor</t> assay of cyclic AMP inhibition. (B) β-arrestin recruitment to the activated KOR was assessed via the Tango assay. (C and D) Results from GloSensor and Tango assays used to calculate bias factors for each compound from observed maximal efficacy (Emax or “Max”) and potency (EC50) values. ∆∆log(Max/EC50) values, with their 95% confidence intervals (CIs), are plotted in (D) for each compound to indicate relative (to U50,488) bias toward G protein signaling. All compounds were tested in triplicate alongside the reference standard U50,488. N ≥ 3 for all compounds.
Pglosensor 22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor-22f+camp+plasmid+dna/pci+expression+vector/pm37220745-178-167-170
Average 90 stars, based on 1 article reviews
pglosensor-22f - by Bioz Stars, 2026-10
90/100 stars
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human genomic dna  (Promega)
90
Promega human genomic dna
Nalfurafine is a potent G protein-biased KOR agonist. Comparison of G protein and β-arrestin signaling outcomes with a variety of KOR ligands (at indicated concentrations) compared with the reference standard U50,488. (A) G protein signaling was assessed with a <t>GloSensor</t> assay of cyclic AMP inhibition. (B) β-arrestin recruitment to the activated KOR was assessed via the Tango assay. (C and D) Results from GloSensor and Tango assays used to calculate bias factors for each compound from observed maximal efficacy (Emax or “Max”) and potency (EC50) values. ∆∆log(Max/EC50) values, with their 95% confidence intervals (CIs), are plotted in (D) for each compound to indicate relative (to U50,488) bias toward G protein signaling. All compounds were tested in triplicate alongside the reference standard U50,488. N ≥ 3 for all compounds.
Human Genomic Dna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor-22f+camp+plasmid+dna/human+genomic+dna/pm37220745-178-15-20
Average 90 stars, based on 1 article reviews
human genomic dna - by Bioz Stars, 2026-10
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celltiter-glo 2.0 cell viability assay  (Promega)
90
Promega celltiter-glo 2.0 cell viability assay
Nalfurafine is a potent G protein-biased KOR agonist. Comparison of G protein and β-arrestin signaling outcomes with a variety of KOR ligands (at indicated concentrations) compared with the reference standard U50,488. (A) G protein signaling was assessed with a <t>GloSensor</t> assay of cyclic AMP inhibition. (B) β-arrestin recruitment to the activated KOR was assessed via the Tango assay. (C and D) Results from GloSensor and Tango assays used to calculate bias factors for each compound from observed maximal efficacy (Emax or “Max”) and potency (EC50) values. ∆∆log(Max/EC50) values, with their 95% confidence intervals (CIs), are plotted in (D) for each compound to indicate relative (to U50,488) bias toward G protein signaling. All compounds were tested in triplicate alongside the reference standard U50,488. N ≥ 3 for all compounds.
Celltiter Glo 2.0 Cell Viability Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor-22f+camp+plasmid+dna/glosensor+substrate/pm37220745-178-136-142
Average 90 stars, based on 1 article reviews
celltiter-glo 2.0 cell viability assay - by Bioz Stars, 2026-10
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Image Search Results


Nalfurafine is a potent G protein-biased KOR agonist. Comparison of G protein and β-arrestin signaling outcomes with a variety of KOR ligands (at indicated concentrations) compared with the reference standard U50,488. (A) G protein signaling was assessed with a GloSensor assay of cyclic AMP inhibition. (B) β-arrestin recruitment to the activated KOR was assessed via the Tango assay. (C and D) Results from GloSensor and Tango assays used to calculate bias factors for each compound from observed maximal efficacy (Emax or “Max”) and potency (EC50) values. ∆∆log(Max/EC50) values, with their 95% confidence intervals (CIs), are plotted in (D) for each compound to indicate relative (to U50,488) bias toward G protein signaling. All compounds were tested in triplicate alongside the reference standard U50,488. N ≥ 3 for all compounds.

Journal: The Journal of Pharmacology and Experimental Therapeutics

Article Title: Preclinical Testing of Nalfurafine as an Opioid-sparing Adjuvant that Potentiates Analgesia by the Mu Opioid Receptor-targeting Agonist Morphine

doi: 10.1124/jpet.118.255661

Figure Lengend Snippet: Nalfurafine is a potent G protein-biased KOR agonist. Comparison of G protein and β-arrestin signaling outcomes with a variety of KOR ligands (at indicated concentrations) compared with the reference standard U50,488. (A) G protein signaling was assessed with a GloSensor assay of cyclic AMP inhibition. (B) β-arrestin recruitment to the activated KOR was assessed via the Tango assay. (C and D) Results from GloSensor and Tango assays used to calculate bias factors for each compound from observed maximal efficacy (Emax or “Max”) and potency (EC50) values. ∆∆log(Max/EC50) values, with their 95% confidence intervals (CIs), are plotted in (D) for each compound to indicate relative (to U50,488) bias toward G protein signaling. All compounds were tested in triplicate alongside the reference standard U50,488. N ≥ 3 for all compounds.

Article Snippet: Quantification of G protein signaling-dependent changes in intracellular cyclic AMP (cAMP) levels was conducted using a GloSensor luciferase-based assay in HEK293T cells transiently transfected with 5 μ g of KOR expression vector DNA (3×HA-hKOR; www.cDNA.org ) and 5 μ g of pGloSensor-22F cAMP biosensor expression vector DNA (Promega, Madison, WI).

Techniques: Comparison, Inhibition